CSIR Central

Avirulent mutants of Macrophomina phaseolina and Aspergillus fumigatus initiate infection in Phaseolus mungo in the presence of phaseolinone;levamisole gives protection

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata


Field	Value

Title	Avirulent mutants of Macrophomina phaseolina and Aspergillus fumigatus initiate infection in Phaseolus mungo in the presence of phaseolinone;levamisole gives protection

Creator	Sett, Suchandra Mishra, Sontosh K Siddique, K A I

Subject	Infectious Diseases and Immunology

Description	To evaluate the role of phaseolinone, a phytotoxin produced by Macrophomina phaseolina, in disease initiation, three nontoxigenic avirulent mutants of the fungus were generated by UV-mutagenesis. Two of them were able to initiate infection in germinating Phaseolus mungo seeds only in the presence of phaseolinone. The minimum dose of phaseolinone required for infection in 30% seedlings was 2·5 μg/ml. A human pathogen, Aspergillus fumigatus was also able to infect germinating seeds of P. mungo in the presence of 5 μg/ml concentration of phaseolinone. Phaseolinone seemed to facilitate infection by A. fumigatus, which is not normally phytopathogenic, by reducing the immunity of germinating seedlings in a nonspecific way. Levamisole, a non-specific immunopotentiator gave protection against infection induced by A. fumigatus at an optimum dose of 50 μg/ml. Sodium malonate prevented the effects of levamisole.

Date	2000

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://www.eprints.iicb.res.in/858/1/JOURNAL_OF_BIOSCIENCES__25_(1)73%2D80;2000[56].pdf Sett, Suchandra and Mishra, Sontosh K and Siddique, K A I (2000) Avirulent mutants of Macrophomina phaseolina and Aspergillus fumigatus initiate infection in Phaseolus mungo in the presence of phaseolinone;levamisole gives protection. Journal of Bioscieces, 25 (1). pp. 73-80.

Relation	http://dx.doi.org/10.1007/BF02985184 http://www.eprints.iicb.res.in/858/