Thyroid Hormone-Induced Morphological Differentiation and Maturation of Astrocytes Are Mediated Through the b-Adrenergic Receptor
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Thyroid Hormone-Induced Morphological Differentiation
and Maturation of Astrocytes Are Mediated Through
the b-Adrenergic Receptor
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| Creator |
Gharami, Kusumika
Das, Sumantra |
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| Subject |
Cell Biology & Physiology
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| Description |
The molecular mechanisms associated with
thyroid hormone (TH)-induced maturation of astrocytes
have been studied using primary cultures. We have previously
demonstrated that unlike normal astrocyte cultures,
hypothyroid cultures fail to differentiate from flat
polygonal cells with epithelioid morphology into mature
process-bearing cells with stellate morphology. Addition
of TH to the hypothyroid cells reverses the effect, and
astrocytes transform into stellate cells. The b-adrenergic
receptor (b-AR) agonist isoproterenol (ISP) has a similar
effect, whereas simultaneous addition of the b-adrenergic
antagonist propranolol blocks the differentiation induced
by TH or ISP. Addition of TH or ISP to hypothyroid
cultures is also associated with a decrease in the level of
filamentous cytoskeletal (Fi) actin and an increase in the
level of actin mRNA. Although addition of propranolol
inhibited the decline in the level of Fi actin in the TH- or
ISP-supplemented cells as well as the induction of actin
mRNA by TH, it partially inhibited the ISP-induced actin
mRNA in these cultures. The hormone-induced maturation
appears to be selectively regulated through the b2-
AR. The overall results indicate that the b-adrenergic
system plays an obligatory role in promoting TH-induced
differentiation and maturation of astrocytes and in regulating
the hormone-induced expression of actin and its
intracellular organization in a way conducive to the morphological
differentiation of the cells. Key Words: Astrocytes—
b-Adrenergic receptors—Hypothyroidism—
Morphology—Actin.
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| Publisher |
John Wiley & Sons
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/861/1/JOURNAL_OF_NEUROCHEMISTRY_75(_5_)1962%2D1969;2000[9].pdf
Gharami, Kusumika and Das, Sumantra (2000) Thyroid Hormone-Induced Morphological Differentiation and Maturation of Astrocytes Are Mediated Through the b-Adrenergic Receptor. Journal of Neurobiology, 75 (5). pp. 1962-1969. ISSN 0022-3034 |
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| Relation |
http://dx.doi.org/10.1046/j.1471-4159.2000.0751962.x
http://www.eprints.iicb.res.in/861/ |
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