Involvement of PL-D in the Alternate Signal Tranduction Pathway of Macrophages induced by an External Stimulus.
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Involvement of PL-D in the Alternate Signal Tranduction Pathway of Macrophages induced by an External Stimulus.
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| Creator |
Bandyopadhyay, Rajyasree
Basu, Mukul K |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
The alternate pathway of signal transduction via hydrolysis of phosphatidylcholine, the major cellular phospholipid, has been
investigated in murine peritoneal macrophages. A sustained formation of diacylglycerol, is preceded by an enhanced production
of phosphatidic acid, when the macrophages were given a stimulus with 12-O-tetradecanoyl phorbol-13-acetate for sixty minutes.
Production of choline and choline metabolites are significantly increased too. Propranolol, which inhibits phosphatidate
phosphohydrolase, the enzyme responsible for conversion of phosphatidic acid to diacylglycerol, can effectively block the
formation of diacylglycerol. Inhibition of protein kinase C either by its inhibitors, staurosporine and H-7 or by depletion, apparently
affect the generation of the lipid products. Moreover, based on the results of transphosphatidylation reaction, involvement of
a phospholipase D in the phosphatidylcholine-hydrolytic pathway in macrophages is predicted. These observations support
the view that probably the phorbol ester acting directly on protein kinase C of the macrophages activate their phosphatidylcholinespecific
phospholipase D to allow a steady generation of second messengers, to enable them to participate in the cell signalling
process in a more efficient manner than those generated in the phosphoinositide pathway of signal transduction.
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| Publisher |
Kluwer
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/871/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY_203(_1%2D2)_127%2D133;2000[70].pdf
Bandyopadhyay, Rajyasree and Basu, Mukul K (2000) Involvement of PL-D in the Alternate Signal Tranduction Pathway of Macrophages induced by an External Stimulus. Molecular and Cellular Biochemistry, 203 (1-2). pp. 127-133. |
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| Relation |
http://dx.doi.org/10.1023/A:1007055804978
http://www.eprints.iicb.res.in/871/ |
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