Ligation of Fc receptor of Macrophages Stimulates Protein Kinase C and Anti-Leishmanial Activity
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive InfoField | Value | |
Title |
Ligation of Fc receptor of Macrophages Stimulates Protein Kinase C and Anti-Leishmanial Activity
|
|
Creator |
Dasgupta, Dalia
Chakraborty, Prasanta Basu, Mukul Kumar |
|
Subject |
Drug Development/Diagnostics & Biotechnology
|
|
Description |
Fc receptors are known to express on the surface of mature monocytes, macrophages and lymphocytes. In this study a ligand
e.g. liposomal IgG (human IgG coupled to PE-liposome via carbodimide reaction) was developed to ligate the Fc receptor of
macrophages. When liposomal IgG was incubated with macrophages at 37°C for 5 min, it induced the macrophage activation
which suppress the parasite burden approximatley to an extent of 60%, 50% and 45%, when macrophages were infected with
UR6, AG83 and GE1 strains of L-donovani respectively. Superior efficacy of liposomal IgG were achieved compared to the
treatment with free IgG and free liposomes. The activity of protein kinase C (PKC) has been found to be higher in the Fc
receptor targeted macrophage membrane fraction, suggesting its translocation from the cytosol. Staurosporine, a potent inhibitor
of the enzyme protein kinase C (PKC) has been found to protect the parasite inside the macrophage indicating the role of PKC
in the signaling process. The liposomal IgG treatment has been found to induce the generation of significant amount of
superoxide and hydrogen peroxide which helped to suppress the parasite burden. Further when liposomal IgG were incubated
with IFN-g primed, LPS activated macrophages, a significant amount of NO release was also noticed, indicating its role in
parasite killing. The above results suggest that Fc receptor mediated activation by liposomal IgG may be used as an alternative
approach to kill parasites intracellularly.
|
|
Date |
2000
|
|
Type |
Article
PeerReviewed |
|
Format |
application/pdf
|
|
Identifier |
http://www.eprints.iicb.res.in/872/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY_209(_1%2D2)_1%2D8;2000[40].pdf
Dasgupta, Dalia and Chakraborty, Prasanta and Basu, Mukul Kumar (2000) Ligation of Fc receptor of Macrophages Stimulates Protein Kinase C and Anti-Leishmanial Activity. Molecular and Cellular Biochemistry, 209 (1-2). 01-08. |
|
Relation |
http://dx.doi.org/10.1023/A:1007051413280
http://www.eprints.iicb.res.in/872/ |
|