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Identification of Thyroid Regulatory Elements in the Na-K-ATPase α3 Gene Promoter

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Identification of Thyroid Regulatory Elements in the Na-K-ATPase α3 Gene Promoter
 
Creator Bajpai, Manish
Mondal, Swapan Kumar
Chaudhury, Sukanya
 
Subject Chemistry
 
Description A −1027 bp to +108 bp region of Na-K-ATPase α3 gene promoter has been searched for the presence of thyroid response elements (TRE). Computer analysis of this sequence using a consensus TRE sequence revealed the presence of four putative TRE rich regions referred to as regions I (−636 to −457 bp), II (−218 to −106 bp), III (−106 to −6 bp) and IV (−6 to +108 bp). Cotransfection of the luciferase linked full length construct as well as constructs progressively devoid of the TRE rich regions in Cos1 cells revealed that regions I and III are positively regulated by T3 whereas there are some sequences in region II which can suppress the positive regulatory effect of region III but not of region I, TRE IV seems to have no functional role. EMSA of the three functional TRE rich regions (I, II and III) showed strong and specific interaction with thyroid hormone receptor (TR) cloned and expressed in baculovirus. The overall results suggest the regulation of Na-K-ATPase α3 gene by T3 is complex involving several thyroidal regulatory elements.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/874/1/MOLECULAR_BIOLOGY_REPORTS%2C28_(_1)%2C_1%2D7%2C2001[54].pdf
Bajpai, Manish and Mondal, Swapan Kumar and Chaudhury, Sukanya (2001) Identification of Thyroid Regulatory Elements in the Na-K-ATPase α3 Gene Promoter. Molecular Biology Reports, 28 (1). pp. 1-7.
 
Relation http://dx.doi.org/10.1023/A:1011986418897
http://www.eprints.iicb.res.in/874/