Generation of ceramide in murine macrophages infected with Leishmania donovani alters macrophage signaling events and aids intracellular parasitic survival
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Generation of ceramide in murine macrophages
infected with Leishmania donovani alters
macrophage signaling events and aids intracellular
parasitic survival
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| Creator |
Ghosh, Sanjukta
Bhattacharyya, Sandip Das, Sonali Raha, Sanghamitra Maulik, Nilanjana Das, Dipak K Roy, Syamal Majumdar, Subrata |
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| Subject |
Chemistry
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| Description |
In the present study, we examined the involvement of intracellular ceramide in host pathogen interaction of BALB/c mouse
peritoneal macrophages infected with the obligate intracellular protozoan, Leishmania donovani. Our findings indicate that
the level of intracellular ceramide was enhanced as a result of the in vitro infection. While the elevated ceramide was largely
due to de novo synthesis, activation of the sphingomyelinases was also observed. The enhanced ceramide was responsible for
the downregulation of classical PKC activity, upregulation of calcium independent atypical PKC-ζ expression and activity of
calcium independent PKC. Ceramide also impaired the phosphorylation of MAPK. Evidently, ceramide suppressed the generation
of nitric oxide during leishmanial infection and also facilitated the survival of leishmanial parasites in the intramacrophageal
milieu. These data present newer insight to the signaling events in leishmania-infected murine macrophages,
which might offer ceramide as a new therapeutic target in the future. (Mol Cell Biochem 223: 47–60, 2001)
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| Date |
2001
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/875/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY%2C__223(_1%2D2)%2C_47%2D60[29].pdf
Ghosh, Sanjukta and Bhattacharyya, Sandip and Das, Sonali and Raha, Sanghamitra and Maulik, Nilanjana and Das, Dipak K and Roy, Syamal and Majumdar, Subrata (2001) Generation of ceramide in murine macrophages infected with Leishmania donovani alters macrophage signaling events and aids intracellular parasitic survival. Molecular and Cellular Biochemistry, 223 (1-2). pp. 47-60. |
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| Relation |
http://dx.doi.org/10.1023/A:1017996609928
http://www.eprints.iicb.res.in/875/ |
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