Paracrine Effects of a Uterine Agglutinin are Mediated via the Sialic Acids Present in the Rat Uterine Endometrium
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Paracrine Effects of a Uterine Agglutinin are
Mediated via the Sialic Acids Present in the Rat
Uterine Endometrium
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| Creator |
Chatterji, U
Sen, A K Schauer, R Chowdhury, Mridula |
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| Subject |
Chemistry
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| Description |
A 32 kDa estrogen-induced, sialic acid-specific agglutinin (P-SAS) was isolated from rat endometrium in its proestrus stage
[1]. To investigate the functional importance of P-SAS in the uterine milieu, specific binding assays were carried out with 125Ilabeled
P-SAS and different cellular components of the uterus (epithelial, stromal and myometrial cells), that were isolated
from different stages of the estrus cycle. The results indicate that although the protein is secreted from the epithelial cells in the
estrogenic phase, it binds specifically to the stromal cells, especially to those isolated from the diestrus stage of the estrus cycle.
The specific binding, however, is seen to decrease with the progression of pregnancy. Scatchard analysis performed with varying
amounts of 125I-P-SAS in the presence of excess cold P-SAS revealed that the binding occurs with a Ka = 1.69 ´ 108 M–1.
As P-SAS binds specifically to sialic acids on the stromal cell surface, further characterization of the sialic acid molecule to
which P-SAS binds was carried out by gas liquid chromatography (GLC). The studies revealed that P-SAS preferentially binds
to N-glycolylneuraminic acid, which is attached to the penultimate sugar of the stromal cell surface glycoprotein chain via
a2,6 linkage. As P-SAS is further known to be mitogenic [2], the effect of P-SAS on cultured stromal cells was studied in
vitro. The growth regulatory assays revealed that P-SAS induced 3H-thymidine uptake by stromal cells in culture. Thus, from
the above observations, paracrine effects of P-SAS on the stromal cells and on the subsequent growth and development of the
uterus can be assumed.
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| Publisher |
Kluwer
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/878/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY_215(1%2D2_)_47%2D55;2000[3].pdf
Chatterji, U and Sen, A K and Schauer, R and Chowdhury, Mridula (2000) Paracrine Effects of a Uterine Agglutinin are Mediated via the Sialic Acids Present in the Rat Uterine Endometrium. Molecular and Cellular Biochemistry, 215 (1-2). pp. 47-55. |
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| Relation |
http://dx.doi.org/10.1023/A:1026582715752
http://www.eprints.iicb.res.in/878/ |
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