Selective Impairment of Protein Kinase C Isotypes in Murine Macrophage by Leishmania Donovani
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Selective Impairment of Protein Kinase C Isotypes
in Murine Macrophage by Leishmania Donovani
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| Creator |
Bhattacharyya, Sandip
Ghosh, Sanjukta Sen, Pradip Roy, Syamal Majumdar, Subrata |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Leishmania donovani, an obligate intracellular parasite resides and multiplies within macrophage of the reticuloendothelial
system. The intracellular signalling mechanism involved in the impaired oxidative response in leishmaniasis has not yet been
clearly established. Generation of superoxide anion (O2
–) is supposed to be the first line of host defence during microbial invasion.
We found a substantial inhibition of superoxide anion generation in parasitized macrophages, which was just the reverse
in case of macrophages challenged with Lipophosphoglycan (LPG) deficient attenuated leishmanial parasite UR-6. The
generation of O2
– essentially needs the prior activation of protein kinase C (PKC) mediated phosphorylation events. Our study
proposed that phosphorylation of 67, 54, 47 and 36 kDa proteins was attenuated during infection. This was supported by PKC
activity study, where Ca-dependent PKC activity was inhibited but, Ca-independent PKC activity was enhanced. This result
was further confirmed by using isotype specific pseudosubstrate inhibitors of Ca-dependent PKC b and Ca-independent PKC
z. Application of b-pseudosubstrate could not alter the Ca-dependent PKC activity but z-pseudosubstrate inhibited the Caindependent
PKC activity in infected macrophages. Our immunoblot analysis with specific antibody against PKC b and PKC
z isotypes showed down regulation of PKC b-II expression with concomitant induction of PKC z. Such inhibition of Ca-dependent PKC b was reversed in macrophages treated with UR-6. Taken together, our observations revealed that infection with L. donovani selectively attenuates both the expression and activity of Ca-dependent PKC b.
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| Date |
2001
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/879/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY%2C_216_(1%2D2)%2C_47%2D57%2C2001_[70].pdf
Bhattacharyya, Sandip and Ghosh, Sanjukta and Sen, Pradip and Roy, Syamal and Majumdar, Subrata (2001) Selective Impairment of Protein Kinase C Isotypes in Murine Macrophage by Leishmania Donovani. Molecular and Cellular Biochemistry, 216 (1-2). pp. 47-57. |
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| Relation |
http://dx.doi.org/10.1023/A:1011048210691
http://www.eprints.iicb.res.in/879/ |
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