Thyroidal Regulation of Different Isoforms of NaKATPase in Glial Cells of Developing Rat Brain
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Thyroidal Regulation of Different Isoforms of NaKATPase
in Glial Cells of Developing Rat Brain
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|
| Creator |
Banerjee, Bhaswati
Choudhuri, Sukanya |
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| Subject |
Chemistry
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| Description |
The developmental profile of the different isoforms of NaKATPase have been investigated during
the first three weeks of postnatal development using primary cultures of isolated glial cells derived
from neonatal rat cerebra. Northern and Western blot analysis show that the expression of four isoforms
(
a
1
,
a
2
,
b
1
and
b
2
) in these cells increases progressively between 5 to 20 days of culture. Comparison
of the mRNA levels of these isoforms in thyroid hormone deficient (TH def) and thyroid hormone supplemented
(TH sup) cells cultured for 5 – 10 days, revealed for the first time that all four isoforms are
sensitive to T 3 in the glial cells. Furthermore immunocytochemical staining of these cells with isoform
specific NaKATPase antibodies also showed that the localization of the different isoforms in the TH
def cells were altered in comparison to that in the TH sup cells. These results establish glial cells as the
target cells for the regulation of NaKATPase by TH in the developing brain. © 2001 Elsevier Science
Inc. All rights reserved.
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| Date |
2001
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/880/1/LIFE_SCIENCES%2C__69_(20)%2C_2409%2D2417%2C2001_[14].pdf
Banerjee, Bhaswati and Choudhuri, Sukanya (2001) Thyroidal Regulation of Different Isoforms of NaKATPase in Glial Cells of Developing Rat Brain. Life Sciences, 69 (20). pp. 2409-2417. |
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| Relation |
http://dx.doi.org/10.1016/S0024-3205(01)01314-5
http://www.eprints.iicb.res.in/880/ |
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