Mutations in a tRNA Import Signal Define Distinct Receptors at the Two Membranes of Leishmania Mitochondria
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Mutations in a tRNA Import Signal Define Distinct Receptors
at the Two Membranes of Leishmania Mitochondria
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| Creator |
Bhattacharyya, Suvendra Nath
Mukherjee, Shankar Adhya, Samit |
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| Subject |
Molecular & Human Genetics
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| Description |
Nucleus-encoded tRNAs are selectively imported into the mitochondrion of Leishmania, a kinetoplastid
protozoan. An oligoribonucleotide constituting the D stem-loop import signal of tRNATyr(GUA) was efficiently
transported into the mitochondrial matrix in organello as well as in vivo. Transfer through the inner membrane
could be uncoupled from that through the outer membrane and was resistant to antibody against the
outer membrane receptor TAB. A number of mutations in the import signal had differential effects on outer and
inner membrane transfer. Some mutants which efficiently traversed the outer membrane were unable to enter
the matrix. Conversely, restoration of the loop-closing GC pair in reverse resulted in reversion of transfer
through the inner, but not the outer, membrane, and binding of the RNA to the inner membrane was restored.
These experiments indicate the presence at the two membranes of receptors with distinct specificities which
mediate stepwise transfer into the mitochondrial matrix. The combination of oligonucleotide mutagenesis and
biochemical fractionation may provide a general tool for the identification of tRNA transport factors.
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| Publisher |
American Society for Microbiology
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
application/pdf |
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| Identifier |
http://www.eprints.iicb.res.in/882/1/MOLECULAR_AND_CELLULAR_BIOLOGY__20_(19_7410%2D7417;2000[16].pdf
http://www.eprints.iicb.res.in/882/2/MOLECULAR_AND_CELLULAR_BIOLOGY__20_(19_7410%2D7417;2000[16].pdf Bhattacharyya, Suvendra Nath and Mukherjee, Shankar and Adhya, Samit (2000) Mutations in a tRNA Import Signal Define Distinct Receptors at the Two Membranes of Leishmania Mitochondria. Molecular and Cellular Biology, 20 (19). pp. 7410-7417. |
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| Relation |
http://dx.doi.org/10.1128/MCB.20.19.7410-7417.2000
http://www.eprints.iicb.res.in/882/ |
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