Temperature-Induced Rapid Increase in Cytoplasmic free Ca 2+ in Pathogenic Leishmania Donovani Promastigotes.
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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Title |
Temperature-Induced Rapid Increase in Cytoplasmic free Ca 2+ in Pathogenic Leishmania Donovani Promastigotes.
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Creator |
Sarkar, Dhiman
Bhaduri, Amar |
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Subject |
Chemistry
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Description |
We demonstrate that the cytoplasmic free Ca 2รท concentration
in three virulent Leishmania donovani promastigote
strains is maintained at 20-30 nM level whereas the aviruleut
promastigotes maintain [Ca2+]i at 80-100 riM. Rapid exposure of
virulent promastigotes to higher physiological temperature increases
[Ca2+]i many-fold. Use of CaCl2, EGTA and Mn 2+ confirmed
that both release of Ca 2+ from internal pool(s) and influx
from outside milieu are responsible for this increase in [Ca2+]|.
Application of oligomycin, CN-, and nigericin indicated the nonmitochondrial
pHi sensitive pool as the possible intracellular origin
for internal Ca 2+ release. Exposure of these cells to acidic
environment had no influence on [Ca2+L. In contrast, the avirulent
promastigotes or freshly isolated amastigotes had shown no effect
of heat-shock or pH shock on [Ca2+li of these cells. Our results
indicate that the upward shift in temperature may play a role in
signal transduction events in morphogenetic transformation of
L. donovani promastigotes that involves mobilization of Ca 2+ in
cytoplasm.
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Publisher |
Elsevier
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Date |
1995
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/891/1/FEBS_LETTERS__375_(1%2D2)_83%2D86;2005[11].pdf
Sarkar, Dhiman and Bhaduri, Amar (1995) Temperature-Induced Rapid Increase in Cytoplasmic free Ca 2+ in Pathogenic Leishmania Donovani Promastigotes. FEBS Letters, 375 (1-2). pp. 83-86. |
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Relation |
http://dx.doi.org/10.1016/0014-5793(95)01161-7 :
http://www.eprints.iicb.res.in/891/ |
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