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Temperature-Induced Rapid Increase in Cytoplasmic free Ca 2+ in Pathogenic Leishmania Donovani Promastigotes.

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Temperature-Induced Rapid Increase in Cytoplasmic free Ca 2+ in Pathogenic Leishmania Donovani Promastigotes.
 
Creator Sarkar, Dhiman
Bhaduri, Amar
 
Subject Chemistry
 
Description We demonstrate that the cytoplasmic free Ca 2รท concentration in three virulent Leishmania donovani promastigote strains is maintained at 20-30 nM level whereas the aviruleut promastigotes maintain [Ca2+]i at 80-100 riM. Rapid exposure of virulent promastigotes to higher physiological temperature increases [Ca2+]i many-fold. Use of CaCl2, EGTA and Mn 2+ confirmed that both release of Ca 2+ from internal pool(s) and influx from outside milieu are responsible for this increase in [Ca2+]|. Application of oligomycin, CN-, and nigericin indicated the nonmitochondrial pHi sensitive pool as the possible intracellular origin for internal Ca 2+ release. Exposure of these cells to acidic environment had no influence on [Ca2+L. In contrast, the avirulent promastigotes or freshly isolated amastigotes had shown no effect of heat-shock or pH shock on [Ca2+li of these cells. Our results indicate that the upward shift in temperature may play a role in signal transduction events in morphogenetic transformation of L. donovani promastigotes that involves mobilization of Ca 2+ in cytoplasm.
 
Publisher Elsevier
 
Date 1995
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/891/1/FEBS_LETTERS__375_(1%2D2)_83%2D86;2005[11].pdf
Sarkar, Dhiman and Bhaduri, Amar (1995) Temperature-Induced Rapid Increase in Cytoplasmic free Ca 2+ in Pathogenic Leishmania Donovani Promastigotes. FEBS Letters, 375 (1-2). pp. 83-86.
 
Relation http://dx.doi.org/10.1016/0014-5793(95)01161-7 :
http://www.eprints.iicb.res.in/891/