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Purification and Characterization of a Growth-Regulating Laccase from Pleurotus Florida

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Purification and Characterization of a Growth-Regulating Laccase from Pleurotus Florida
 
Creator Das, Nirmalendu
Chakraborty, Tapas Kumar
Mukherjee, Mina
 
Subject Chemistry
 
Description Pleurotus florida produces two laccase enzymes (L1 and L2) within which L2 is associated with the vegetative growth of the fungus. In the present investigation the L2 has been purified to homogeneity and characterized. The molecular mass of the enzyme has been determined to be 73 kDa and 70 kDa by gel filtration chromatography and SDS-PAGE, respectively. The purified enzyme shows a pI value of 4.2. The optimum reaction temperature is 50 �C. Spectroscopic analysis reveals that L2 has two copper atoms, a type I copper and a type II copper. The Km and some other kinetic parameters of L2 has been determined.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/905/1/JOURNAL_OF_BASIC_MICROBIOLOGY%2C_41(_5)%2C_261%2D267%2C2001_[66].pdf
Das, Nirmalendu and Chakraborty, Tapas Kumar and Mukherjee, Mina (2001) Purification and Characterization of a Growth-Regulating Laccase from Pleurotus Florida. Journal of Basic Microbiology, 41 (5). pp. 261-267.
 
Relation http://dx.doi.org/10.1002/1521-4028(200110)41:5<261::AID- JOBM261>3.0.CO;2-V
http://www.eprints.iicb.res.in/905/