Purification and Characterization of a Growth-Regulating Laccase from Pleurotus Florida
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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Title |
Purification and Characterization of a
Growth-Regulating Laccase from Pleurotus Florida
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Creator |
Das, Nirmalendu
Chakraborty, Tapas Kumar Mukherjee, Mina |
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Subject |
Chemistry
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Description |
Pleurotus florida produces two laccase enzymes (L1 and L2) within which L2 is associated with the
vegetative growth of the fungus. In the present investigation the L2 has been purified to homogeneity
and characterized. The molecular mass of the enzyme has been determined to be 73 kDa and 70 kDa
by gel filtration chromatography and SDS-PAGE, respectively. The purified enzyme shows a pI value
of 4.2. The optimum reaction temperature is 50 �C. Spectroscopic analysis reveals that L2 has two
copper atoms, a type I copper and a type II copper. The Km and some other kinetic parameters of L2
has been determined.
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Date |
2001
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/905/1/JOURNAL_OF_BASIC_MICROBIOLOGY%2C_41(_5)%2C_261%2D267%2C2001_[66].pdf
Das, Nirmalendu and Chakraborty, Tapas Kumar and Mukherjee, Mina (2001) Purification and Characterization of a Growth-Regulating Laccase from Pleurotus Florida. Journal of Basic Microbiology, 41 (5). pp. 261-267. |
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Relation |
http://dx.doi.org/10.1002/1521-4028(200110)41:5<261::AID- JOBM261>3.0.CO;2-V
http://www.eprints.iicb.res.in/905/ |
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