O-acetyl sialic acid specific IgM in childhood acute lymphoblastic leukaemia
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
O-acetyl sialic acid specific IgM in childhood
acute lymphoblastic leukaemia
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| Creator |
Pal, Santanu
Chatterjee, Mitali Bhattacharya, Dilip K Bandyopadhyay, Santu Mandal, Chhabinath Mandal, Chitra |
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| Subject |
Cell Biology & Physiology
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| Description |
Initial studies have revealed an enhanced surface expression of O-acetylated sialoglycoconjugates (O-AcSGs) on lymphoblasts
concomitant with high titres of IgG in childhood Acute Lymphoblastic Leukaemia (ALL) (Mandal C, Chatterjee
M, Sinha D, Br J Haematol 110, 801–12, 2000). In our efforts to identify disease specific markers for ALL, we have affinitypurified
IgM directed against O-AcSGs that reacts with three disease specific O-AcSGs present on membrane proteins
derived from peripheral blood mononuclear cells (PBMC) of ALL patients. Antibody specificity towards O-AcSGs was
confirmed by selective binding to erythrocytes bearing surface O-AcSGs, decreased binding with de-O-acetylated BSM
and following pretreatment with O-acetyl esterase. Competitive inhibition ELISA demonstrated a higher avidity of IgM for
O-AcSG than IgG. Flow cytometry demonstrated the diagnostic potential of purified O-AcSA IgM as binding was specific
with ALL patients and minimal with other haematological disorders and normal individuals. It therefore may be adopted as a non-invasive approach for detection of childhood ALL. Taken together, the data indicates that carbohydrate epitopes having terminal O-AcSA α2→6 GalNAc determinants induce disease specific IgG and IgM, potentially useful molecular
markers for childhood ALL.
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| Date |
2001
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/911/1/GLYCOCONJUGATE_JOURNAL%2C18(_7)%2C_529%2D537[30].pdf
Pal, Santanu and Chatterjee, Mitali and Bhattacharya, Dilip K and Bandyopadhyay, Santu and Mandal, Chhabinath and Mandal, Chitra (2001) O-acetyl sialic acid specific IgM in childhood acute lymphoblastic leukaemia. Glycoconjugate Journal, 18 (7). pp. 529-537. |
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| Relation |
http://dx.doi.org/10.1023/A:1019692329568
http://www.eprints.iicb.res.in/911/ |
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