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O-acetyl sialic acid specific IgM in childhood acute lymphoblastic leukaemia

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title O-acetyl sialic acid specific IgM in childhood acute lymphoblastic leukaemia
Creator Pal, Santanu
Chatterjee, Mitali
Bhattacharya, Dilip K
Bandyopadhyay, Santu
Mandal, Chhabinath
Mandal, Chitra
Subject Cell Biology & Physiology
Description Initial studies have revealed an enhanced surface expression of O-acetylated sialoglycoconjugates (O-AcSGs) on lymphoblasts concomitant with high titres of IgG in childhood Acute Lymphoblastic Leukaemia (ALL) (Mandal C, Chatterjee M, Sinha D, Br J Haematol 110, 801–12, 2000). In our efforts to identify disease specific markers for ALL, we have affinitypurified IgM directed against O-AcSGs that reacts with three disease specific O-AcSGs present on membrane proteins derived from peripheral blood mononuclear cells (PBMC) of ALL patients. Antibody specificity towards O-AcSGs was confirmed by selective binding to erythrocytes bearing surface O-AcSGs, decreased binding with de-O-acetylated BSM and following pretreatment with O-acetyl esterase. Competitive inhibition ELISA demonstrated a higher avidity of IgM for O-AcSG than IgG. Flow cytometry demonstrated the diagnostic potential of purified O-AcSA IgM as binding was specific with ALL patients and minimal with other haematological disorders and normal individuals. It therefore may be adopted as a non-invasive approach for detection of childhood ALL. Taken together, the data indicates that carbohydrate epitopes having terminal O-AcSA α2→6 GalNAc determinants induce disease specific IgG and IgM, potentially useful molecular markers for childhood ALL.
Date 2001
Type Article
Format application/pdf
Identifier http://www.eprints.iicb.res.in/911/1/GLYCOCONJUGATE_JOURNAL%2C18(_7)%2C_529%2D537[30].pdf
Pal, Santanu and Chatterjee, Mitali and Bhattacharya, Dilip K and Bandyopadhyay, Santu and Mandal, Chhabinath and Mandal, Chitra (2001) O-acetyl sialic acid specific IgM in childhood acute lymphoblastic leukaemia. Glycoconjugate Journal, 18 (7). pp. 529-537.
Relation http://dx.doi.org/10.1023/A:1019692329568