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Cholera Toxin (CTX) Genetic Element in Vibrio cholerae 0139

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Cholera Toxin (CTX) Genetic Element in Vibrio cholerae 0139
Creator Bhadra, Rupak K
Roychoudhury, Susanta
Banerjee, Rajat K
Kar, Sujata
Majumdar, Ruma
Sengupta, Sanghamitra
Chatterjee, Soma
Khetawat, GopaI
Das, Jyotirmoy
Subject Infectious Diseases and Immunology
Molecular & Human Genetics
Description PFGE analysis of the Notl- and Sfil-digested genome of Virbrio cholerae 0139 strains isolated from different epidemic regions of India showed that all the strains are of clonal origin and the genome size is about 2.2 Mb. An analysis of the electrophoretic profiles of the genome of 0139 strains, the RFLP of the cholera toxin (age)ne and Southern blot hybridization of Notidigested genomes of classical, El Tor and 0139 with a Noti-linking clone of classical strain 5698, suggest that these strains closely resemble V. cholerae 01 biotype El Tor, but are widely different from the classical 01 vibrios. Using restriction enzymes which cleave a single site in either the core region or in the direct repeat sequence (RS) of the CTX genetic element, it has been shown that the genome of most of the 0139 strains has two copies of the ctx gene in tandem connected by two RSs. The chromosomal location of the CTX genetic element in the 0139 strain is the same as that reported for El Tor vibrios. The organization of the virulence gene cassettes in different 0139 strains shows genetic heterogeneity in the population. Whilst most of the epidemic strains have two copies of the CTX genetic element, in some strains the number of elements has been amplified and in at least one strain a single copy of the element has been deleted.
Date 1995
Type Article
Format application/pdf
Identifier http://www.eprints.iicb.res.in/913/1/MICROBIOLOGY%2DUK__141%2C__1977%2D1983;1995[30].pdf
Bhadra, Rupak K and Roychoudhury, Susanta and Banerjee, Rajat K and Kar, Sujata and Majumdar, Ruma and Sengupta, Sanghamitra and Chatterjee, Soma and Khetawat, GopaI and Das, Jyotirmoy (1995) Cholera Toxin (CTX) Genetic Element in Vibrio cholerae 0139. Microbiology, 141. pp. 1977-1983.
Relation http://dx.doi.org/10.1099/13500872-141-8-1977