Identification of 9-O-acetylated sialoglycans on peripheral blood mononuclear cells in Indian Visceral Leishmaniasis
IR@IICB: CSIR-Indian Institute of Chemical Biology, KolkataView Archive Info
Identification of 9-O-acetylated sialoglycans
on peripheral blood mononuclear cells in Indian
Although the existence of O-acetylated sialic acids is well known, it is only in recent years that steady refinement of
analytical techniques have enabled detailed mapping of their structural diversity . Fluorimetric analysis of peripheral
blood mononuclear cells (PBMC) of patients with Visceral Leishmaniasis (VL) showed six fold increase in the percentage
of surface 9-O-acetylated sialoglycoconjugates (9-O-AcSGs) as compared to normal human donors. Using Achatinin-H,
a 9-O-acetyl sialic acid- binding lectin, an enhanced presence of 9-O-AcSGs in an α2→6 linkage was demonstrated by
flow cytometry; abolition of its binding by pretreatment with a recombinant 9-O-acetylesterase corroborated the presence
of this glycotope. Western blotting of PBMC from VL patients indicated the presence of five O-acetylated sialoglycans corresponding to 144, 65, 56, 36 and 19 kDa as compared to 144 and 36 kDa in normal individuals. Taken together our data indicates that during active disease, there is an overexpression of 9-O-AcSGs on the surface of PBMC of VL patients, thus opening up new research avenues wherein the expression of this biomarker could be exploited to monitor the clinical status of VL patients.
Bandyopadhyay, Sumi and Chatterjee, Mitali and Sundar, Shyam and Mandal, Chitra (2003) Identification of 9-O-acetylated sialoglycans on peripheral blood mononuclear cells in Indian Visceral Leishmaniasis. Glycoconjugate Journal, 20 (9). pp. 531-536.