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A novel sarsasapogenin glycoside from Asparagus racemosus elicits protective immune responses against HBsAg

IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu

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Title A novel sarsasapogenin glycoside from Asparagus racemosus elicits protective immune responses against HBsAg
 
Creator Sidiq, Tabasum
Khajuria, Anamika
Suden, Pankaj
Singh, Surjeet
Satti, N.K.
Suri, K.A.
Srinivas, V.K.
Krishna, E.
Johri, R.K.
 
Subject Pharmacological Sciences
 
Description The aim of the present investigation was to evaluate the adjuvant potential of a novel sarsasapogenin glycoside (immunoside) isolated from Asparagus racemosus in combination with hepatitis B surface antigen(HBsAg). Various in vitro and animal derived protocols were used to determine the response of immunoside adjuvanted with HBsAg and the results were compared with alum adjuvanted with HBsAg.Several biomarkers such as antibody titre (IgG, IgG1/IgG2a) were measured in mice sera. Cell proliferation, cytokines (IL-2, IFN-� and IL-4), and lymphocyte sub-populations (CD4/CD8, CD3 and CD19)were determined in splenocytes from mice administered subcutaneously with test substances. In these cells CD4/CD8 derived IFN-� release was also determined. Macrophage preparations were used for the determination of IL-12, IFN-� and nitrite content. Seroconversion potential was compared with a standard vaccine. Acute safety evaluation of immunoside was done in mice. Effect of immunoside on red blood cell haemolysis was determined. The results have suggested that immunoside potentially enhanced anti-HBsAg immune response via augmenting Th1/Th2 response in a dose dependent manner.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/192/1/10.1016j.imlet.2010.10.013.pdf
Sidiq, Tabasum and Khajuria, Anamika and Suden, Pankaj and Singh, Surjeet and Satti, N.K. and Suri, K.A. and Srinivas, V.K. and Krishna, E. and Johri, R.K. (2011) A novel sarsasapogenin glycoside from Asparagus racemosus elicits protective immune responses against HBsAg. Immunology Letters, 135 (1-2). pp. 129-135. ISSN 01652478
 
Relation http://dx.doi.org/10.1016/j.imlet.2010.10.013
http://iiim.csircentral.net/192/