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Genetic transformation of Vitreoscilla sp.

IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh

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Title Genetic transformation of Vitreoscilla sp.
 
Creator Navani, Naveen K
Joshi, M
Dikshit, Kanak L
 
Subject QH426 Genetics
 
Description Of all the methods customarily used to transform E. coli we found only electroporation to be effective for transformation of the Gram-negative bacterium Vitreoscilla, yielding 5.10(5) transformants/microgram of plasmid DNA. The conditions used were close to those described for E. coli E. coli plasmids are stably maintained in Vitreoscilla. This is the first report of exogenous DNA transfer in Vitreoscilla which opens the way for the application of recombinant-DNA techniques to study this unique group of organisms.
 
Publisher Elsevier Science
 
Date 1996-10-24
 
Type Article
PeerReviewed
 
Relation http://www.sciencedirect.com/science/article/pii/0378111996002843
http://crdd.osdd.net/open/375/
 
Identifier Navani, Naveen K and Joshi, M and Dikshit, Kanak L (1996) Genetic transformation of Vitreoscilla sp. Gene, 177 (1-2). pp. 265-6. ISSN 0378-1119