Sites of intermolecular crosslinking of fatty acyl chains in phospholipids carrying a photoactivable carbene precursor
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Gupta, C M
Costello, Catherine E Khorana, H Govind |
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| Date |
2008-03-23T07:09:05Z
2008-03-23T07:09:05Z 1979 |
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| Identifier |
Proc. Natl. Acad. Sci. USA (1979), 76, 3139-3143
http://hdl.handle.net/123456789/115 |
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| Description |
Sonicated vesicles of l-fatty acyl-2-w-(2-diazo-3.3,3-trifluoropropionoxy) fatty acyl sn-glycero-3-phosphorylcholines were shown recently to form intermolecular crosslinks
by insertion of the photogenerated carbene into a C-H bond of a neighboring hydrocarbon chain. We now report that photolysis of multilamellar dispersions gives a second series of products in which carbene insertion is accompanied by elimination of a molecule of hydrogen fluoride. The sites of crosslinking
in the latter compounds have been studied by mass spectrometry using phospholipids with varying chain lengths of the fatty acyl groups carrying the carbene precursor. The patterns observed show that the point of maximum crosslinking is consistent with the recent conclusion that in phospholipids
the sn-2 fatty acyl chain trails the sn-1 chain by 2-4 atoms.
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| Format |
3156800 bytes
application/pdf |
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| Language |
en
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| Title |
Sites of intermolecular crosslinking of fatty acyl chains in phospholipids carrying a photoactivable carbene precursor
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| Type |
Article
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