Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
View Archive Info| Field | Value | |
| Title |
Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity
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| Creator |
Chaubey, Asha
Parshad, Rajinder Koul, Surrinder Taneja, Subhash C. Qazi, Ghulam N. |
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| Subject |
Biological Sciences
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| Description |
Arthrobacter sp. lipase (ABL, MTCC no. 5125)is being recognized as an efficient enzyme for the
resolution of drugs and their intermediates. The immobilization of ABL on various matrices for its enantioselectivity,stability, and reusability has been studied. Immobilization by covalent bonding on sepharose and silica afforded a maximum of 380 and 40 IU/g activity, respectively,whereas sol 13gel entrapment provided a maximum of 150 IU/g activity in dry powder. The immobilized enzyme
displayed excellent stability in the pH range of 4 1310 and
even at higher temperature, i.e., 50 1360
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/218/1/10.1007s00253%2D006%2D0520%2D5.pdf
Chaubey, Asha and Parshad, Rajinder and Koul, Surrinder and Taneja, Subhash C. and Qazi, Ghulam N. (2006) Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity. Applied Microbiology and Biotechnology, 73 (3). pp. 598-606. ISSN 0175-7598 |
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| Relation |
http://dx.doi.org/10.1007/s00253-006-0520-5
http://iiim.csircentral.net/218/ |
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