CSIR Central

Enzymatic resolution of naproxen

IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu

View Archive Info
 
 
Field Value
 
Title Enzymatic resolution of naproxen
 
Creator Koul, Surrinder
Parshad, Rajinder
Taneja, Subhash C.
Qazi, Ghulam N.
 
Subject Chemical Sciences
 
Description Trichosporon sp. (TSL), a newly found strain isolated from a locally fermented cottage cheese has been found to be highly stereoselective in the resolution of(S)-(+)-naproxen (ee >99%, E�500) from the corresponding racemic methyl ester. The process of resolution using whole cells has been scaled up to multi-kg level. Optimization of experimental conditions including downstream processing at 80–100 g/L substrate concentration with >90% recovery has been achieved. Changes in the physical parameters such as the particle size of the substrate play an important role in the resolution kinetics. A new strain of Trichosporon sp. having high cell density in cultivation (>60 g dry cell mass L−1 in 14–16 h) is found to be sufficiently stable for two years in dry powder form at 5–8°C. The viability of the resolution process has been further improved by the development of a simple racemization process for the enriched (R)-(−)-ester.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/251/1/10.1016S0957%2D4166(03)00492%2D0.pdf
Koul, Surrinder and Parshad, Rajinder and Taneja, Subhash C. and Qazi, Ghulam N. (2003) Enzymatic resolution of naproxen. Tetrahedron: Asymmetry, 14 (16). pp. 2459-2465. ISSN 09574166
 
Relation http://dx.doi.org/10.1016/S0957-4166(03)00492-0
http://iiim.csircentral.net/251/