Evaluation of cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
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| Title |
Evaluation of cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
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| Creator |
Bhagat, Madhulika
Saxena, AjitKumar |
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| Subject |
Pharmacological Sciences
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| Description |
Objective : To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains.
Material and Methods : In vitro cytotoxicity against the human cancer cells, cultured for 48h in presence of different concentrations C. occidentalis extracts and percentage of cell viability, was evaluated using the sulforhodamine-B (SRB) assay. The antibacterial activity was performed using the standard protocol against bacterial strains.
Results : It was observed that aqueous extract of C. occidentalis (whole plant) had more potential than hydro-alcoholic and alcoholic extracts against HCT-15, SW-620, PC-3, MCF-7, SiHa, and OVCAR-5 human cancer cell lines at 100, 30, and 10 μg/ml in a dose-dependent manner. The hydro-alcoholic extract showed potential against Bacillus subtillis.
Conclusion : The plant can be explored for the possible development of lead molecules for drug discovery.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/254/1/10.4103%2D0253%2D7613.68428.pdf
Bhagat, Madhulika and Saxena, AjitKumar (2010) Evaluation of cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity. Indian Journal of Pharmacology, 42 (4). p. 234. ISSN 0253-7613 |
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| Relation |
http://dx.doi.org/10.4103/0253-7613.68428
http://iiim.csircentral.net/254/ |
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