Selective Th1 upregulation by ethyl acetate fraction of Labisia pumila
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
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| Title |
Selective Th1 upregulation by ethyl acetate fraction of Labisia pumila
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| Creator |
Pandey, Anjali
Bani, Sarang Sangwan, Payarelal Koul, Surrinder |
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| Subject |
Pharmacological Sciences
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| Description |
Aim of the study: Labisia pumila has rejuvenating properties that help women to regain the strength and
vigour after giving birth (Burkill, 1935) leading us to speculate that Labisia pumila might also modulate the immune matrix as it is one of the important aspect of healthy state of the living body.
Materials and methods: Studies were carried out for the evaluation of pro-inflammatory cytokines in murine neutrophils followed by expression of T cell-surface markers and corresponding intracellular expression of cytokines related to T helper1 (Th1) and T helper 2 (Th2) activity.
Results: The ethyl acetate fraction (A001/3b) obtained from ethanolic extract of leaves of Labisia pumila at graded doses (in vitro) showed increased expression of TNF-�, IL-6 and IL-12. IL-12 is a strong inducer of Th1 response in activated CD4+ T cells and accordingly T cell studies were carried out (in vivo). These specific studies showed A001/3b to cause marked stimulation of CD4+ T helper cells and related cytokine expression like IL-2 and IFN-gamma (a potent Th1 inducer), thereby, having a predominant Th1 upregulation pathway. This was further validated by suppression of IL-4 and IL-10 expression which are the
Th2 pathway cytokines.
Conclusion: This study showed ethyl acetate fraction (A001/3b) of Labisia pumila to have Th1 upregulating
activity and suggests its possible usefulness as a therapeutic agent in immune compromised patients.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/338/1/10.1016j.jep.2010.08.039.pdf
Pandey, Anjali and Bani, Sarang and Sangwan, Payarelal and Koul, Surrinder (2010) Selective Th1 upregulation by ethyl acetate fraction of Labisia pumila. Journal of Ethnopharmacology, 132 (1). pp. 309-315. ISSN 03788741 |
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| Relation |
http://dx.doi.org/10.1016/j.jep.2010.08.039
http://iiim.csircentral.net/338/ |
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