Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
View Archive Info| Field | Value | |
| Title |
Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay
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| Creator |
Kumar, Manoj
Khan, Inshad Ali Verma, Vijashoor Kalyan, Narender Qazi, Giulam Nabi |
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| Subject |
Biological Sciences
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| Description |
A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination.
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/328/1/10.1016j.diagmicrobio.2005.05.011.pdf
Kumar, Manoj and Khan, Inshad Ali and Verma, Vijashoor and Kalyan, Narender and Qazi, Giulam Nabi (2005) Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay. Diagnostic Microbiology and Infectious Disease, 53 (2). pp. 121-124. ISSN 07328893 |
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| Relation |
http://dx.doi.org/10.1016/j.diagmicrobio.2005.05.011
http://iiim.csircentral.net/328/ |
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