Flavone Glycoside Based Validated RP-LC Method for Quality Evaluation of Prishniparni (Uraria picta)
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
View Archive Info| Field | Value | |
| Title |
Flavone Glycoside Based Validated RP-LC Method for Quality Evaluation of Prishniparni (Uraria picta)
|
|
| Creator |
Yadav, Akhilesh K.
Yadav, Deepti Shanker, Karuna Verma, Ram K. Saxena, Ajit K. Gupta, Madan M. |
|
| Subject |
Chemical Sciences
|
|
| Description |
An LC method is developed for the quantitation of rhoifolin in Uraria picta, a plant of high usage frequency in all Asian traditional systems of medicine. An isocratic RP-LC method using C18 column, UV detection 265 nm and specificity with PDA and MS is speeding up, reliable
and comprehensive analysis of rhoifolin in U. picta. Good linearity was obtained in the working range (0.02–0.10 mg mL-1), with correlation coefficients >0.99. LOD and LOQ
were 2.33 and 7.69 ng, respectively. The method was validated following international guidelines. The described method can be utilized for assays and stability tests of U. picta extracts as well as Ayurvedic drugs based on Prishniparni.
|
|
| Date |
2009
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://iiim.csircentral.net/376/1/10.1365s10337%2D009%2D0963%2D9.pdf
Yadav, Akhilesh K. and Yadav, Deepti and Shanker, Karuna and Verma, Ram K. and Saxena, Ajit K. and Gupta, Madan M. (2009) Flavone Glycoside Based Validated RP-LC Method for Quality Evaluation of Prishniparni (Uraria picta). Chromatographia, 69 (7-8). pp. 653-658. ISSN 0009-5893 |
|
| Relation |
http://dx.doi.org/10.1365/s10337-009-0963-9
http://iiim.csircentral.net/376/ |
|