Studies on cytotoxic, hydroxyl radical scavenging and topoisomerase inhibitory activities of extracts of Tabernaemontana divaricata (L.) R.Br. ex Roem. and Schult
IR@IIIM: CSIR-Indian Institute of Integrative Medicine, Jammu
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| Title |
Studies on cytotoxic, hydroxyl radical scavenging and topoisomerase inhibitory activities of extracts of Tabernaemontana divaricata (L.) R.Br. ex Roem. and Schult
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| Creator |
Thind, Tarunpreet Singh
Agrawal, Satyam Kumar Saxena, A.K. Arora, Saroj |
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| Subject |
Pharmacological Sciences
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| Description |
In the present investigation, the cytotoxic, hydroxyl radical scavenging and topoisomerase inhibition activities of Tabernaemontana divaricata (Apocynaceae) were evaluated. The extracts from leaves of the plant were prepared with different solvents viz. chloroform, methanol, ethyl acetate and hexane. In, invitro cytotoxicity assay, with cell lines viz HCT-15 (Colon), HT-29 (Colon), 502713 (Colon), MCF-7
(Breast), PC- 3 (Prostrate), it was observed that the ethyl acetate extract was effective against only onecolon cell line (502713) at the lowest dose i.e. 10 lg/ml, whereas the chloroform extract was effective against all the three colon cancer cell lines, at 30 lg/ ml. In order to evaluate the mechanism of cytotoxicity of these extracts, they were assessed for their ability to scavenge hydroxyl radicals in plasmid nicking assay with pBR322. It was observed that all the extracts effectively inhibited the unwinding of
supercoiled DNA except hexane extract, which showed the least effect. Since the expression of topo enzymes is linked with cell proliferation so the extracts were also checked for topo I and topo II inhibitory activities. It was noticed that ethyl acetate extract selectively showed inhibition of topo II in topoisomerase II relaxation assay.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://iiim.csircentral.net/346/1/10.1016j.fct.2008.05.036.pdf
Thind, Tarunpreet Singh and Agrawal, Satyam Kumar and Saxena, A.K. and Arora, Saroj (2008) Studies on cytotoxic, hydroxyl radical scavenging and topoisomerase inhibitory activities of extracts of Tabernaemontana divaricata (L.) R.Br. ex Roem. and Schult. Food and Chemical Toxicology, 46 (8). pp. 2922-2927. ISSN 02786915 |
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| Relation |
http://dx.doi.org/10.1016/j.fct.2008.05.036
http://iiim.csircentral.net/346/ |
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