CSIR Central

Spectropolarimetric Determination of the Guanine-Cytosine Content of DNA

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

View Archive Info
 
 
Field Value
 
Title Spectropolarimetric Determination of the Guanine-Cytosine Content of DNA
 
Creator Maiti, Motilal
Nandi, R
 
Subject Chemistry
 
Description A circular dichroism technique for the determination of DNA base composition in terms of the guanine-cytosine content has been developed. A linear relationship between the ratio of ellipticities at 260 and 280 nm (S,,/6&,) and base composition of DNA is obtained in citratephosphate buffer, pH 3.0 at 20°C. The DNA base composition obtained with this method is not only highly reproducible but is also the fastest method reported.
 
Date 1987
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1420/1/ANALYTICAL_BIOCHEMISTRY___164(_1)_68%2D71;1987[33].pdf
Maiti, Motilal and Nandi, R (1987) Spectropolarimetric Determination of the Guanine-Cytosine Content of DNA. Analytical BIiochemistry, 164 (1). pp. 68-71.
 
Relation http://dx.doi.org/10.1016/0003-2697(87)90368-X
http://www.eprints.iicb.res.in/1420/