Phosphatidyl Inosito Inhibition Of a Sperm Cyclic Amp-Independent Protein Kinase
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Phosphatidyl Inosito Inhibition Of a Sperm
Cyclic Amp-Independent Protein Kinase
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| Creator |
Dey, Chinmoy S.
Majumder, Gopal Chandra |
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| Subject |
Cell Biology & Physiology
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| Description |
Phosphatidyl inositol has been found to inhibit strongly the activity of
a cyclic AMP-independent protein kinase located on the external surface of goat epididymal
intact spermatozoa. Phosphatidyl inositol at a concentration as low ai 10 &/ml
inhibited nearly 50% of the ecto-kinase activity for the phosphorylation of the exogenous
protein substrate: casein. Phosphatidyl ethanolamine at a relatively high concentration
(125 &ml) inhibited slightly (approx 25%) the activity of the enzyme whereas other
phospholipids: phosphatidyl serine and choline, diacyl glycerol, phosphatidic acid and
myo-inositol-2-phosphate had no appreicable effect on the kinase activity. Phosphatidyl
inositol has also served as a potent inhibitor of the phosphorylation of sperm ectophosphoproteins
by the endogenous kinase activity of intact spermatozoa. By thin
layer chromatography it has been shown that the observed inhibitory effect of the
phospholipid was not due to any impurities or degraded products of phosphatidyl inositol.
Phosphatidyl iy$tol inhibited the kinase activity noncompetitively with respect to
casein and Mg but uncompetitively with respect to ATP. The results raised the
possibility that phosphatidyl inositol-mediated high affinity inhibition of protein kinase(s),
may constitute a novel mechanism for the regulatory actions of the phospholipid in mammalian cells.
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| Publisher |
Elsevier
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| Date |
1987
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1423/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS__146(_2);_1987[29].pdf
Dey, Chinmoy S. and Majumder, Gopal Chandra (1987) Phosphatidyl Inosito Inhibition Of a Sperm Cyclic Amp-Independent Protein Kinase. Biochemical and Biophysical Research Communications, 146 (2). pp. 422-429. |
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| Relation |
http://dx.doi.org/10.1016/0006-291X(87)90546-8
http://www.eprints.iicb.res.in/1423/ |
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