Liposome as an Adjuvant for the Production of Estradiol Antibodies
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Liposome as an Adjuvant for the Production of Estradiol Antibodies
|
|
| Creator |
Ali, Nahid
Mandal, Chitra |
|
| Subject |
Infectious Diseases and Immunology
|
|
| Description |
Mice immunized with liposome associated
estradiol-bovine serum albumin conjugate
(E2-BSA) showed a significantly higher estradiolspecific
immune response than did mice injected
with the free conjugate. The conjugation of estradiol
with BSA is imperative, since injection of liposomes
with estradiol in the lipid bilayer was found
to be ineffective in inducing an immune response.
Entrapment of E2-BSA in neutral and positively
charged liposomes was found to be a potent mode
of immunostimulation, even better than emulsification
in complete Freund's adjuvant (CFA). Covalent
liposome surface association of E2-BSA induced
a good immunopotentiation. However, the
response was lower than that observed with the entrapped
conjugate. The affinity and the specificity
of antisera, raised in different ways, were determined.
|
|
| Publisher |
Elsevier
|
|
| Date |
1987
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/1432/1/IMMUNOLOGY_LETTERS__15(3)_237%2D242;1987[34].pdf
Ali, Nahid and Mandal, Chitra (1987) Liposome as an Adjuvant for the Production of Estradiol Antibodies. Immunology Letters, 15 (3). pp. 237-242. |
|
| Relation |
http://dx.doi.org/10.1016/0169-328X(87)90009-X
http://www.eprints.iicb.res.in/1432/ |
|