Acetylcholinesterase activity in marine gastropods as biomarker of neurotoxic contaminants
IR@NIO: CSIR-National Institute Of Oceanography, Goa
View Archive Info| Field | Value | |
| Creator |
Sarkar, A.
Gaitonde, D.C.S. Vashistha, D. |
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| Date |
2012-08-24T07:52:22Z
2012-08-24T07:52:22Z 2011 |
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| Identifier |
In "Environmental pollution: Ecological impacts, health issues and management. Eds. by: Bhattacharya, B.; Ghosh, A.; Majumdar, S.K.Mudrakar"; Kolkata; India; 2011; 100-107p.
http://drs.nio.org/drs/handle/2264/4129 |
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| Description |
The acetylcholinesterase (AchE) activity was measured in three different species of marine gastropods namely Cronia contracta, Morula granulata and Planaxis sulcatus collected from selected stations (Arambol, Anjuna, Dona Paula, Vasco and Velsao) along the Goa coast for evaluation of the impact of neurotoxicants. The AChE activity was measured following the delta pH-metric method using acetylcholine bromide as the substrate and bromothymol blue as the indicator in phosphate buffer. The AChE activity was expressed as the micro moles of acetic acid liberated per mg of proteins per minute and shown in term of arbitrary units. The AChE activity was compared with respect to that from a relatively uncontaminated region (Anjuna) along the Goa coast. Among the different species of the marine gastropod studied Morula granulata was found to be most predominant followed by Cronia contracta and Planaxis sulcatus. All the species of marine gastropods showed highest AChE activity (Morula granulata, 51.09 units, Cronia contracta, 22.73 units, and Planaxis sulcatus, 10.17 units) at Anjuna. The AChE activity was found to be lowest at Dona Paula in respect of Morula granulata (30.04 unit) and Planaxis sulcatus (5.28 units) whereas in the case of Cronia contracta it was at Vasco (12.77 units). Along the Goa coast the AChE activities increased in the order: Dona Paula less than Vasco less than Velsao less than Arambol less than Anjuna. The increase in AChE activities along the Goa coast clearly indicated the extent of contamination of the coastal environment by neurotoxic contaminants.
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| Language |
en
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| Publisher |
Mudrakar and Institute of Ecotoxicology and Environmental Sciences
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| Rights |
©Dr. Badal Bhattacharya, President,
Institute of Ecotoxicology & Environmental Sciences. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
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| Subject |
pollution detection
toxicity neurotransmitters Cronia contracta Morula granulata Planaxis sulcatus |
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| Title |
Acetylcholinesterase activity in marine gastropods as biomarker of neurotoxic contaminants
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| Type |
Book chapter
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