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Leishmania donovani Parasites Interact with g/dþ Human Peripheral Blood T Cells and Induce Susceptibility to NK Cell-Mediated Lysis

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata


Field	Value

Title	Leishmania donovani Parasites Interact with g/dþ Human Peripheral Blood T Cells and Induce Susceptibility to NK Cell-Mediated Lysis

Creator	Saha, A Chakrabarty, G Sen, S Bandyopadhyay, Santu

Subject	Infectious Diseases and Immunology

Description	We recently reported that Leishmania donovani infect the human T-cell line in vitro. To examine whether primary human T cells could be infected by this parasite, a direct interaction of the peripheral blood T cells with L. donovani was examined. The percentage of g/dþ T cells was markedly increased when in vitro generated normal human T-cell blasts were cultured with L. donovani amastigotes. About 30% of the g/dþ T cells in the parasite exposed T-cell blasts expressed parasite antigens intracellularly without detectable intracellular parasites. Parasite exposed T-cell blasts had a reduced surface expression of HLA-DR and were lysed by the sorted CD56þ cells. In contrast, neither L. donovani amastigotes nor T-cell blasts exposed to heat killed amastigotes and/or were sensitive to the NK cell-mediated lysis. Of interest is that about 10% CD3þ peripheral blood T cells in two out of three Indian Kala-azar patients tested expressed intracellular L. donovani antigens.

Publisher	Blackwell Publishing

Date	1999

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://www.eprints.iicb.res.in/1635/1/Scand_J._Immunol%2C_1999.pdf Saha, A and Chakrabarty, G and Sen, S and Bandyopadhyay, Santu (1999) Leishmania donovani Parasites Interact with g/dþ Human Peripheral Blood T Cells and Induce Susceptibility to NK Cell-Mediated Lysis. Scandinavian Journal Of Immunology, 50. pp. 588-595.

Relation	http://dx.doi.org/10.1046/j.1365-3083.1999.00642.x http://www.eprints.iicb.res.in/1635/