Visceral Leishmaniasis in India: Promises and Pitfalls of a PCR-Based Blood Test
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Visceral Leishmaniasis in India: Promises and Pitfalls of a PCR-Based Blood Test
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| Creator |
Adhya, Samit
Hassan, Md Quamarul Mukherjee, Sandeep Manna, Partha Pratim Basu, Anirban Sen, Sandeep Bandyopadhyay, Santu |
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| Subject |
Infectious Diseases and Immunology
Molecular & Human Genetics |
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| Description |
Traditional methods of diagnosing visceral leishmaniasis (kala-azar) in India suffer from a number of
disadvantages. Amplification of multicopy nuclear genes and messenger ribonucleic acid of Leishmania
by the polymerase chain reaction (PCR) was evaluated as an alternative assay under various clinical
conditions. PCR of peripheral blood has the highest absolute sensitivity among all the available
procedures, and is particularly useful for detecting parasites in early infections, post kala-azar dermal
leishmaniasis, concurrent infections and immunocompromised cases, but is not so reliable for late
infections. PCR of immunopurified blood mononuclear cells indicated the association of parasites with
monocytes as well as non-monocyte cell types.
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| Publisher |
Elsevier
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| Date |
2002
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1640/1/Trans._Royal_Society_of_Trop._Med._and_Hygiene%2C_2002.pdf
Adhya, Samit and Hassan, Md Quamarul and Mukherjee, Sandeep and Manna, Partha Pratim and Basu, Anirban and Sen, Sandeep and Bandyopadhyay, Santu (2002) Visceral Leishmaniasis in India: Promises and Pitfalls of a PCR-Based Blood Test. Transactions of The Royal Society of Tropical Medicine and Hygiene, 96. S1/179-S1/183. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/1640/ |
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