A novel recombinant Leishmania donovani p45, a partial coding region of methionine aminopeptidase, generates protective immunity by inducing a Th1 stimulatory response against experimental visceral leishmaniasis
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
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| Creator |
Gupta, Reema
Kushawaha, P K Tripathi, C D P Sundar, Shyam Dube, Anuradha |
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| Date |
2012-10-03T11:54:49Z
2012-10-03T11:54:49Z 2012 |
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| Identifier |
International Journal for Parasitology , 2012, 42, (5), 429–435
http://hdl.handle.net/123456789/923 |
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| Description |
The development of a vaccine against visceral leishmaniasis (VL) conferring long-lasting immunity remains a challenge. Identification and proteomic characterization of parasite proteins led to the detection of p45, a member of the methionine aminopeptidase family. To our knowledge the present study is the first known report that describes the molecular and immunological characterization of p45. Recombinant Leishmania donovani p45 (rLdp45) induced cellular responses in cured hamsters and generated Th1-type cytokines from peripheral blood mononuclear cells of cured/endemic VL patients. Immunization with rLdp45 exerted considerable prophylactic efficacy (~85%) supported by an increase in mRNA expression of iNOS, IFN-γ, TNF-α and IL-12 and decrease in TGF-β and IL-4, indicating its potential as a vaccine candidate against VL.
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| Format |
1562191 bytes
application/pdf |
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| Language |
en
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| Relation |
CDRI Communication No. 8215
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| Subject |
Leishmania donovani
Recombinant p45 Methionine aminopeptidase Immunogenicity T-cell response Cured Leishmania hamsters Cured Leishmania patients Endemic contacts Prophylactic potential |
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| Title |
A novel recombinant Leishmania donovani p45, a partial coding region of methionine aminopeptidase, generates protective immunity by inducing a Th1 stimulatory response against experimental visceral leishmaniasis
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| Type |
Article
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