Identilfication of Membrane Antigens of Goat Epididym Spermatozoa
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Identilfication of Membrane Antigens of Goat Epididym Spermatozoa
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| Creator |
Chatterjee, Tapati
Majumder, Gopal Chandra |
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| Subject |
Cell Biology & Physiology
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| Description |
Purified goat sperm plasma membrane was used as antigen to raise
the antibody in rabbit. ?Lsing this antisera four groups of antigenic membrane
polypeptides are determined in caput and cauda epididymal sperm. The immunoresponsiveness
of the polypeptides in caput and cauda sperm differs significantly.
In case of cauda epididymal sperm, the polypeptides of region A (96ma,
82KDa, 78KDa, 6SKDa) and region D (24KDa, 2OKDa, 1SRDa) are highly immunoresponsive
whereas in case of caput epididymal sperm the same antisera recognized
the polypeptides of region R, C and D. By surface labelling with lactoperoxidase
iodination and subsequent immunoprecipitation in the iodinated cell extract
we demonstrate eight of these above polypeptides (96KDa, 82KDa, 6SJCDa, SOKDa,
29KDa, 24KDa, 2OKDa and 1SKDa) as surface antigen. The 96KDa, R2RDa and 6WDa
surface polypeptides are highly immunoresponsive than the other lower molecular
weight surface antigens in cauda epididymal goat spermatozoa.
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| Publisher |
Elsevier
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| Date |
1989
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1644/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS__Volume_162___Issue_1___Pages_550%2D556__%2C1989[23].pdf
Chatterjee, Tapati and Majumder, Gopal Chandra (1989) Identilfication of Membrane Antigens of Goat Epididym Spermatozoa. Biochemical and Biophysical Research Communications, 162 (1). pp. 550-556. |
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| Relation |
http://dx.doi.org/10.1016/0006-291X(89)92032-9
http://www.eprints.iicb.res.in/1644/ |
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