A Mutation in the dam Gene of Vibrio-Cholerae - 2-Aminopurine Sensitivity with Intact Gatc Methylase Activity
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
A Mutation in the dam Gene of Vibrio-Cholerae - 2-Aminopurine Sensitivity with Intact Gatc Methylase Activity
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| Creator |
Bandyopadhyay, Rupa
Sengupta, Aditya Das, Jyotirmoy |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Vibrio cholerae mutants sensitive to 2-aminopurine (2AP) but with DNA adenine
methylase activity similar to parental cells have been isolated, The mutant
strains were sensitive to ultraviolet light (UV), methyl methane sulphonate (MMS)
and 9-aminoacridine. The spontaneous mutation frequency of the mutants were
not significantly affected, Attempts to isolate dam- V, cholerae cells by screening
2AP sensitive cells have not been successful, All the mutant phenotypes
could be suppressed by introducing the plasmid pRB103 carrying the dam gene
of Escherichia coli into the mutant cells.
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| Publisher |
Elsevier
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| Date |
1989
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1645/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS__Volume_165___Issue_2___Pages_561%2D567_1989[1].pdf
Bandyopadhyay, Rupa and Sengupta, Aditya and Das, Jyotirmoy (1989) A Mutation in the dam Gene of Vibrio-Cholerae - 2-Aminopurine Sensitivity with Intact Gatc Methylase Activity. Biochemical and Biophysical Research Communications, 165 (2). pp. 561-567. |
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| Relation |
http://dx.doi.org/10.1016/S0006-291X(89)80003-8
http://www.eprints.iicb.res.in/1645/ |
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