Purification of Thiobacillus Ferrooxidans Cultures by Single Colony Isolation and Influences of Agarose on the Colony Morphology
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Purification of Thiobacillus Ferrooxidans Cultures by Single Colony Isolation and Influences of Agarose on the Colony Morphology
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| Creator |
Das, A
Bhattacharyya, Saswati Banerjee, P C |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
Purification of Thiobacillusferrooxidans cultures by single colony isolation has been a real problem partly
because of the difficulty to grow the cells on a solid medium. As a result many apparently pure cultures
of T. ferrooxidans have been found to contain more than one strain or acidophilic heterotrophic bacteria
as contaminants. Adoption of an agarose-based solid medium to grow this organism appears dependable
either to distinguish the strains according to colony morphology or to purify a strain from heterotrophic
contaminants by single colony isolation. That colony morphology of the same strain may differ depending
on the source of agarose is another interesting observation which advises one to maintain strictly identical
growth conditions to distinguish strains according to colony morphology.
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| Publisher |
Elsevier
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| Date |
1989
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1650/1/JOURNAL_OF_MICROBIOLOGICAL_METHODS__Volume_10___Issue_4___Pages_281%2D287%2C1989[6].pdf
Das, A and Bhattacharyya, Saswati and Banerjee, P C (1989) Purification of Thiobacillus Ferrooxidans Cultures by Single Colony Isolation and Influences of Agarose on the Colony Morphology. Journal of Microbiological Methods, 10. pp. 281-287. |
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| Relation |
http://dx.doi.org/10.1016/0167-7012(89)90015-8
http://www.eprints.iicb.res.in/1650/ |
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