Enzymeless creatinine estimation using poly(3,4-ethylenedioxythiophene)-beta-cyclodextrin
IR@CECRI: CSIR-Central Electrochemical Research Institute, Karaikudi
View Archive Info| Field | Value | |
| Title |
Enzymeless creatinine estimation using poly(3,4-ethylenedioxythiophene)-beta-cyclodextrin
|
|
| Creator |
Nareshkumar, T.
Ananthi, A. Mathiyarasu, J. Joseph, J. Phani, K.L.N. Yegnaraman, V. |
|
| Subject |
Electrodics and Electrocatalysis
|
|
| Description |
A novel enzymeless biosensor has been constructed for the quantitative estimation of creatinine using bcyclodextrin
(bCD) incorporated poly-3,4-ethylenedioxythiophene (PEDOT) modified glassy carbon electrode (GCE). The PEDOT film with and without bCD was deposited on a GCE by electropolymerization of EDOT from an aqueous solution containing lithium perchlorate and bCD. Polymer films were characterized by UV–visible spectroscopy, cyclic voltammetry and scanning electron microscopy (SEM). The selective
interactions of the bCD-incorporated PEDOT film with creatinine in neutral Tris buffer solutions were
elucidated using electrochemical impedance analysis. Complex formation between bCD and creatinine was inferred from regular shifts in the electrode potential versus creatinine concentration. In the potentiometric evaluation, the biosensor electrode exhibited a fast response time (60 s), linear shift in the potential over a concentration range of 10^4 10^1 M with a lower detection limit of 5*10^5 M and good stability, retaining nearly 95% of the initial response after 1 month of shelf life. The results of the studies
on the interferences from glucose, ascorbic acid, uric acid, urea and ammonium ions are also presented
|
|
| Publisher |
Elsevier
|
|
| Date |
2011
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://cecri.csircentral.net/2901/1/078-2011.pdf
Nareshkumar, T. and Ananthi, A. and Mathiyarasu, J. and Joseph, J. and Phani, K.L.N. and Yegnaraman, V. (2011) Enzymeless creatinine estimation using poly(3,4-ethylenedioxythiophene)-beta-cyclodextrin. Journal of Electroanalytical Chemistry, 661 (002). pp. 303-308. ISSN 1572-6657 |
|
| Relation |
http://cecri.csircentral.net/2901/
|
|