An Improved Protocol for the Isolation of RNA from Roots of Tea (Camellia sinensis (L.) O. Kuntze)
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
An Improved Protocol for the Isolation of RNA from Roots of Tea
(Camellia sinensis (L.) O. Kuntze)
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| Creator |
Muoki, R C
Paul, A Kumari, A Singh, K Kumar, Sanjay |
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| Subject |
Plant sciences
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| Description |
Tea, a beverage crop, is a rich source of polyphenols
and polysaccharides which greatly attribute to its
importance. However, oxidation and precipitation of these
compounds during nucleic acids extraction is a limitation
to molecular biology and genomic studies. On isolation
of total RNA from root tissue using established protocols,
difficulties were encountered in terms of purity and
quantity of isolated RNA or some of the methods were
time-consuming and also yields were low. The present
communication combines a phenol-based RNA isolation
protocol with a cetyltrimethylammonium bromide-based
procedure with appropriate modifications. This protocol
successfully isolated RNA from tap root tissue in 2–3 h as
compared with 16 h reported by the previous method. Also,
RNA yield was higher by more than fourfold. The RNA
isolated by this protocol was successfully used for downstream
applications such as RT-PCR and the construction
of suppression subtractive hybridization library. The
developed protocol worked well with other plant tissue
with high polyphenols and polysaccharides contents.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1100/1/33.pdf
Muoki, R C and Paul, A and Kumari, A and Singh, K and Kumar, Sanjay (2012) An Improved Protocol for the Isolation of RNA from Roots of Tea (Camellia sinensis (L.) O. Kuntze). Molecular Biotechnology, 52 (1). pp. 82-88. |
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| Relation |
http://ihbt.csircentral.net/1100/
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