A simple method to assess bacterial attachment to surfaces
IR@NIO: CSIR-National Institute Of Oceanography, Goa
View Archive Info| Field | Value | |
| Creator |
Sonak, S.
Bhosle, N.B. |
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| Date |
2009-01-25T05:42:31Z
2009-01-25T05:42:31Z 1995 |
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| Identifier |
Biofouling, Vol.9; 31-38p.
http://hdl.handle.net/2264/2312 |
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| Description |
The crystal violet microplate adhension assay was modified to evaluate bacterial adhesion to metal and non-metal surfaces. Both viable cell count and the absorbance of the crystal violet stained cells attached to aluminium increased over the period of ineubation. There was a highly significant positive linear relationship between crystal violet stained attached cells and the viable cell count of cells attached to aluminium panels (r = 0.9997; p less than 0.001: n = 6). The method is relatively simple, sensitive, less time consuming and therefore many samples can be analysed in a short period of time. When the calibrated method was employed to assess the attachment of Vibrio sp to polystyrene, stainless steel and copper, it give a fairly reliable estimate of bacterial adhension to these surfaces
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| Language |
en
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| Publisher |
Herwood Academic
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| Rights |
Copyright [1995]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
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| Subject |
biological settlement
microorganisms aluminium |
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| Title |
A simple method to assess bacterial attachment to surfaces
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| Type |
Journal Article
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