Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae)
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Identification and validation of sex-linked SCAR markers
in dioecious Hippophae rhamnoides L. (Elaeagnaceae)
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| Creator |
Korekar, Girish
Sharma, R K Kumar, Rahul ......,, Meenu Bisht, Naveen C. Srivastava , Ravi B Ahuja, Paramvir Singh Stobdan, Tsering |
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| Subject |
Plant sciences
Plant Biotechnology |
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| Description |
The actinorhizal plant seabuckthorn
(Hippophae rhamnoides L., Elaeagnaceae) is a wind
pollinated dioecious crop. To distinguish male genotypes
from female genotypes early in the vegetative
growth phase, we have developed robust PCR-based
marker(s). DNA bulk samples from 20 male and 20
female plants each were screened with 60 RAPD
primers. Two primers, OPA-04 and OPT-06 consistently
amplified female-specific (FS) polymorphic
fragments of 1,164 and 868 bp, respectively, that
were absent in the male samples. DNA sequence of the
two markers did not exhibit significant similarity to
previously characterized sequences. A sequence-characterized
amplified region marker HrX1 (JQ284019)
and HrX2 (JQ284020) designed for the two fragments,
continued to amplify the FS allele in 120 female plants
but not in 100 male plants tested in the current study.
Thus, HrX1 and HrX2 are FS markers that can
determine the sex of seabuckthorn plants in an
early stage and expedite cultivations for industrial
applications.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1067/1/106_Identification_and_validation_2012.pdf
Korekar, Girish and Sharma, R K and Kumar, Rahul and ......,, Meenu and Bisht, Naveen C. and Srivastava , Ravi B and Ahuja, Paramvir Singh and Stobdan, Tsering (2012) Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae). Biotechnol Lett. |
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| Relation |
http://ihbt.csircentral.net/1067/
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