Changes in sugar levels during slow growth of Dendrocalamus hamiltonii somatic embryos due to liquid paraffin overlay
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Changes in sugar levels during slow growth
of Dendrocalamus hamiltonii somatic embryos
due to liquid paraffin overlay
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| Creator |
Kaur, D
Ogra, R K Bhattacharya, Amita Sood, Anil |
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| Subject |
Plant Biotechnology
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| Description |
A liquid paraffin overlay (LPO) was used for
storage of rapidly multiplying somatic embryos of
Dendrocalamus hamiltonii under slow-growth conditions.
Slow growth was associated with changes in sugar
metabolism. In rapidly growing embryogenic tissues, a
sharp decline in starch and non-reducing sugars indicated
rapid utilization of starch. In contrast, under slow-growth
conditions in somatic embryos stored under LPO, a
gradual decline in starch indicated its slower utilization.
As a result, growth of somatic embryos under LPO was
suppressed and subculturing was not required. Following
retrieval from growth under LPO after 30, 90, 180, 270,
and 365 d of storage, the somatic embryos showed high
recovery and germination (79.78%, 77.49%, 71.22%,
67.13%, and 59.99%, respectively) and were able to
proliferate following transfer to Murashige and Skoog’s
(Physiol. Plant. 15:473–497, 1962) medium containing
1 mgl−1 BAP and 2% sucrose. The study provides useful
information on in vitro storage of embryogenic tissue of
D. hamiltonii.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1205/1/8_Changes.pdf
Kaur, D and Ogra, R K and Bhattacharya, Amita and Sood, Anil (2012) Changes in sugar levels during slow growth of Dendrocalamus hamiltonii somatic embryos due to liquid paraffin overlay. In Vitro Cellular and Developmental Biology - Plant, 48. pp. 120-126. |
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| Relation |
http://ihbt.csircentral.net/1205/
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