CsNAM-like protein encodes a nuclear localized protein and responds to varied cues in tea [Camellia sinensis (L.) O. Kuntze]
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive InfoField | Value | |
Title |
CsNAM-like protein encodes a nuclear localized protein and responds to varied cues in
tea [Camellia sinensis (L.) O. Kuntze]
|
|
Creator |
Paul, A
Muoki, R C Singh, K Kumar, Sanjay |
|
Subject |
Plant sciences
Plant Biotechnology |
|
Description |
Abiotic stress possesses serious threat to plant distribution and production. In response to stress, plants induce
the expression of many genes that function to protect the cellular machinery from stress-induced damages.
These genes are largely regulated by specific transcription factors (TFs). NAC family proteins are plant
specific TFs implicated in diverse processes including development, and biotic and abiotic stress responses.
The present work described (i) cloning of CsNAM-like protein gene from a tree crop tea [Camellia sinensis
(L.) O. Kuntze], (ii) its cellular localization, and (iii) regulation of the gene by external cues. The gene had
an open reading frame of 873 base pairs encoding 291 amino acids with calculated molecular weight of
33.4 kDa and an isoelectric point (pI) of 6.72. Expression characterization showed the gene to be induced
by drought, osmoticum, salt, heat and hydrogen peroxide. During the period of active growth, CsNAM-like
protein showed ubiquitous expression in all the tissues analyzed, with higher level of transcripts in stem,
flower bud and mature leaf as compared to the root, young leaf and fruit. The common response of
CsNAM-like protein to various cues suggests its important role in imparting tolerance against abiotic stress.
|
|
Date |
2012
|
|
Type |
Article
PeerReviewed |
|
Format |
application/pdf
|
|
Identifier |
http://ihbt.csircentral.net/1206/1/9_CsNAM%2Dlike.pdf
Paul, A and Muoki, R C and Singh, K and Kumar, Sanjay (2012) CsNAM-like protein encodes a nuclear localized protein and responds to varied cues in tea [Camellia sinensis (L.) O. Kuntze]. Gene, 502. pp. 69-74. |
|
Relation |
http://ihbt.csircentral.net/1206/
|
|