Engineering a thermo-stable superoxide dismutase functional at sub-zero to .50uC, which also tolerates autoclaving
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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Title |
Engineering a thermo-stable superoxide
dismutase functional at sub-zero to
.50uC, which also tolerates autoclaving
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Creator |
Kumar, A
Dutt, Som Bagler, Ganesh Ahuja, Paramvir Singh Kumar, Sanjay |
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Subject |
Plant sciences
Natural Product Chemistry |
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Description |
Superoxide dismutase (SOD) is a critical enzyme associated with controlling oxygen toxicity arising out of
oxidative stress in any living system. A hyper-thermostable SOD isolated from a polyextremophile higher
plant Potentilla atrosanguinea Lodd. var. argyrophylla (Wall. ex Lehm.) was engineered by mutation of a
single amino acid that enhanced the thermostability of the enzyme to twofold. The engineered enzyme was
functional from sub-zero temperature to .506C, tolerated autoclaving (heating at 1216C, at a pressure of
1.1 kg per square cm for 20 min) and was resistant to proteolysis. The present work is the first example to
enhance the thermostability of a hyper-thermostable protein and has potential to application to other
proteins for enhancing thermostability.
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Date |
2012
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://ihbt.csircentral.net/1216/1/20_Engineering.pdf
Kumar, A and Dutt, Som and Bagler, Ganesh and Ahuja, Paramvir Singh and Kumar, Sanjay (2012) Engineering a thermo-stable superoxide dismutase functional at sub-zero to .50uC, which also tolerates autoclaving. Scientific Reports (Nature Publishing Group), 2. p. 387. |
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Relation |
http://ihbt.csircentral.net/1216/
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