Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze
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| Creator |
Rani, A
Singh, K Ahuja, Paramvir Singh Kumar, Sanjay |
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| Subject |
Plant sciences
Plant Biotechnology |
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| Description |
Catechins are bioprospecting molecules present in tea and any effort towards metabolic engineering of this
important moiety would require knowledge on gene regulation. These are synthesized through the activities
of phenylpropanoid and flavonoid pathways. Expression regulation of various genes of these pathways namely
phenylalanine ammonia-lyase (CsPAL), cinnamate 4-hydroxylase (CsC4H), p-coumarate:CoA ligase (Cs4CL), flavanone
3-hydroxylase (CsF3H), dihydroflavonol 4-reductase (CsDFR) and anthocyanidin reductase (CsANR) was
accomplished previously. In depth analyses of the remaining genes namely, chalcone synthase (CsCHS), chalcone
isomerase (CsCHI), flavonoid 3′5′-hydroxylase (CsF3′5′H) and anthocyanidin synthase (CsANS) were lacking. The
objective of the work was to clone and analyze these genes so as to generate a comprehensive knowledge on
the critical genes of catechins biosynthesis pathway. Gene expression analysis was carried out in response to
leaf age and external cues (drought stress, abscisic acid, gibberellic acid treatments and wounding). A holistic
analysis suggested that CsCHI, CsF3H, CsDFR, CsANS and CsANR were amongst the critical regulatory genes in
regulating catechins content.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1222/1/27_Molecular.pdf
Rani, A and Singh, K and Ahuja, Paramvir Singh and Kumar, Sanjay (2012) Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze. Gene, 495. pp. 205-210. |
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| Relation |
http://ihbt.csircentral.net/1222/
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