Overexpression of Camellia sinensis Thaumatin-Like Protein, CsTLP in Potato Confers Enhanced Resistance to Macrophomina phaseolina and Phytophthora infestans Infection
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Overexpression of Camellia sinensis Thaumatin-Like Protein,
CsTLP in Potato Confers Enhanced Resistance to Macrophomina
phaseolina and Phytophthora infestans Infection
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| Creator |
Acharya , K
Pal, A.K. Gulati, Arvind Kumar, Sanjay Singh, Anil Kumar Ahuja, Paramvir Singh |
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| Subject |
Plant sciences
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| Description |
Thaumatin-like proteins (TLPs), a class of
pathogenesis related proteins are induced in response to
pathogens and exhibit antifungal property when overexpressed
in transgenic plants. In the present study, we have
raised transgenic potato plants overexpressing TLP gene of
Camellia sinensis (CsTLP). Fungal resistance assays of
transgenic potato elucidated the potential role of CsTLP in
imparting tolerance to fungal pathogens, Macrophomina
phaseolina (necrotrophic) and Phytophthora infestans
(hemi-biotrophic). Transgenic tubers with higher resistance
to M. phaseolina, showed a concomitant and significant
increase in transcripts of StPAL, StLOX, and StTLP genes
involved in phenylpropanoid, lipoxygenase, and general
defense response pathway, respectively after infection.
Importantly, leaves of CsTLP transgenic lines inoculated
with P. infestans spores under in vitro conditions also
showed a resistant phenotype. The resistant phenotype
recorded for the two important fungal pathogens by CsTLP
transgenic potato plants is remarkable, since no effective
control methods and no resistant cv. against M. phaseolina
has been identified so far in potato.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1227/1/31_Over_expression.pdf
Acharya , K and Pal, A.K. and Gulati, Arvind and Kumar, Sanjay and Singh, Anil Kumar and Ahuja, Paramvir Singh (2012) Overexpression of Camellia sinensis Thaumatin-Like Protein, CsTLP in Potato Confers Enhanced Resistance to Macrophomina phaseolina and Phytophthora infestans Infection. Molecular Biotechnology. |
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| Relation |
http://ihbt.csircentral.net/1227/
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