DNA biosensor for detection of Neisseria gonorrhoeae causing sexually transmitted disease
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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Title |
DNA biosensor for detection of Neisseria gonorrhoeae causing
sexually transmitted disease
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Creator |
Singh, Renu
Sumana, G. Verma, Rachna Sood, Seema Pandey, M. K. Gupta, Rajinder K. Malhotra, B. D. |
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Subject |
Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology Mathematics Medical Laboratory Technology |
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Description |
A sequence-specific electrochemical sexually transmitted disease (STD) sensor based on self-assembled monolayer of thiolated DNA probe specific to target opa gene for detection of Gonorrhoea, a sexually transmitted disease has been fabricated. 6-Mercapto-1-hexanol (MCH) has been used as a blocking agent to facilitate oligos “stand” up at the surface, a configuration favoring subsequent DNA hybridization and to repel non-specific adsorption of undesired DNA. The results of differential pulse voltammetric studies of this STD sensor reveal low detection limit (1.0×10−18 M) and a wide dynamic range (from 1.0×10−6M to 0.5×10−18 M) arising due to the stable hybridization using methylene blue as an electro-active DNA
hybridization indicator. The experimental results with genomic DNA, clinical patient sample of Neisseria
gonorrhoeae, culture of non-N. gonorrhoeae Neisseria species (NgNS) and gram negative bacteria indicate
that the fabricated sensor is specific to this STD.
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Publisher |
Elsevier
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Date |
2010-09-10
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://npl.csircentral.net/88/1/63.pdf
Singh, Renu and Sumana, G. and Verma, Rachna and Sood, Seema and Pandey, M. K. and Gupta, Rajinder K. and Malhotra, B. D. (2010) DNA biosensor for detection of Neisseria gonorrhoeae causing sexually transmitted disease. Journal of Biotechnology, 150 (3). pp. 357-365. ISSN 0168-1656 |
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Relation |
http://dx.doi.org/10.1016/j.jbiotec.2010.09.935
http://npl.csircentral.net/88/ |
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