DNA hybridization on silicon nanowires
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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Title |
DNA hybridization on silicon nanowires
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Creator |
Singh, Shalini
Zack , Jyoti Kumar, Dinesh Srivastava , S. K. -, Govind Saluja , Daman Khan, M. A. Singh , P. K. |
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Subject |
Materials Science
Physics |
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Description |
Nanowire-based detection strategies provide promising new routes to bioanalysis and indeed are attractive to conventional systems because of their small size, high surface-to-volume ratios, electronic, and optical
properties. A sequence-specific detection of single-stranded oligonucleotides using silicon nanowires
(SiNWs) is demonstrated. The surface of the SiNWs is functionalized with densely packed organic monolayer via hydrosilylation for covalent attachment. Subsequently, deoxyribonucleic acid (DNA) is immobilized to recognize the complementary target DNA. The biomolecular recognition properties of the nanowires are tested via hybridization with γP32 tagged complementary and non-complementary DNA oligonucleotides, showing good selectivity and reversibility. No significant non-specific binding to the incorrect sequences is observed. X-ray photoelectron spectroscopy, fluorescence imaging, and nanodrop techniques are used to characterize the modified SiNWs and covalent attachment with DNA. The results show that SiNWs are
excellent substrates for the absorption, stabilization and detection of DNA sequences and could be used for
DNA microarrays and micro fabricated SiNWs DNA sensors.
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Publisher |
Elsevier
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Date |
2010-08-20
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://npl.csircentral.net/89/1/64.pdf
Singh, Shalini and Zack , Jyoti and Kumar, Dinesh and Srivastava , S. K. and -, Govind and Saluja , Daman and Khan, M. A. and Singh , P. K. (2010) DNA hybridization on silicon nanowires. Thin Solid Films, 519 (3). pp. 1151-1155. ISSN 0040-6090 |
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Relation |
http://dx.doi.org/10.1016/j.tsf.2010.08.060
http://npl.csircentral.net/89/ |
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