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Electrochemical Impedance Immunosensor for the Detection of C-Reactive Protein in Aqueous Solution

IR@NPL: CSIR-National Physical Laboratory, New Delhi


Field	Value

Title	Electrochemical Impedance Immunosensor for the Detection of C-Reactive Protein in Aqueous Solution

Creator	-, Rajesh Sharma, Vikash Tanwar, Vinod Kumar Biradar, A. M.

Subject	Chemistry Electrochemistry Instruments/ Instrumentation Physics

Description	A label-free electrochemical impedance immunosensor is reported by immobilizing protein antibody, αCRP-Ab, through a self assembled monolayer (SAM) of 3-aminopropyltriethoxysilane (APTES) using a cross linker, Bis[sulfosuccinimidyl] suberate (BS3), on indium tin oxide (ITO) coated glass electrode. The immunosensor (αCRP-Ab/BS3/APTES/ITOglass) was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical techniques. The electrochemical performance of the immunosensor was studied by electrochemical impedance spectroscopy. The results showed an increasing electron-transfer resistance with the immobilization of CRP antibody (αCRP-Ab) on the modified ITO coated glass electrode surface and on their coupling with protein CRP antigen (αCRP-Ag) at the immunosensor surface in the presence of [Fe(CN)6]3−/4− as redox probe. The immunosensor exhibits an electrochemical impedance response to antigen, αCRP-Ab, concentrations in a linear range from 8.5 ng to 9.12 μg mL−1 a with a lowest detection limit of 3.5 ng mL−1 antigen.

Publisher	American Scientific Publishers

Date	2010-04

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://npl.csircentral.net/119/1/93.pdf -, Rajesh and Sharma, Vikash and Tanwar, Vinod Kumar and Biradar, A. M. (2010) Electrochemical Impedance Immunosensor for the Detection of C-Reactive Protein in Aqueous Solution. Sensor Letters, 8 (2). pp. 362-369. ISSN 1546-198X

Relation	http://dx.doi.org/10.1166/sl.2010.1278 http://npl.csircentral.net/119/