Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection
|
|
| Creator |
Pandey, Chandra Mouli
Sumana, Gajjala Sood, K. N. Malhotra, B. D. |
|
| Subject |
Materials Science
Physics |
|
| Description |
An electrochemical DNA biosensor based on cystine modified self-assembled monolayer (cys-SAM) onto gold electrode (AuE) has been fabricated for Escherichia coli (E. coli) detection. This biosensing electrode has been characterized using scanning electron microscopy (SEM), FT-IR spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, this DNA biosensor can be used to detect complementary target DNA concentration in the range of 1 × 10− 6 M to 1 × 10− 20 M within 60 s of hybridization time at 25 °C and has been found to be stable for about four months when stored at 4 °C.
|
|
| Publisher |
Elsevier
|
|
| Date |
2010-09
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://npl.csircentral.net/122/1/96.pdf
Pandey, Chandra Mouli and Sumana, Gajjala and Sood, K. N. and Malhotra, B. D. (2010) Electrochemical studies of cystine modified self-assembled monolayer for Escherichia coli detection. Thin Solid Films, 519 (3). pp. 1178-1183. ISSN 0040-6090 |
|
| Relation |
http://dx.doi.org/10.1016/j.tsf.2010.08.065
http://npl.csircentral.net/122/ |
|