Electrophoretically deposited nano-structured polyaniline film for glucose sensing
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Electrophoretically deposited nano-structured polyaniline film for glucose sensing
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| Creator |
Dhand, Chetna
Sumana, G. Datta, Monika Malhotra, B. D. |
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| Subject |
Materials Science
Physics |
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| Description |
Electrophoretically deposited nano-structured polyaniline (NS-PANI) film has been utilized for fabrication of glucose biosensor by covalent immobilization of glucose oxidase (GOx) using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. This GOx/NS-PANI/ITO bioelectrode has been characterized using scanning electron microscopy, FT-IR, UV–Visible spectroscopy and differential pulse voltammetry (DPV) techniques. The response studies carried out on GOx/NS-PANI/ITO bioelectrode using DPV and photometric studies reveal linearity up to 400 mgdL− 1 with sensitivity as 1.05 × 10− 4 mA mg− 1 dL and 3.887 × 10− 5 Abs mg− 1 dL, respectively. The lower value of Michaelis–Menten constant obtained for immobilized GOx (2.1 mM) compared with that of free GOx (5.85 mM) suggests high affinity of enzyme to this matrix.
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| Publisher |
Elsevier
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| Date |
2010-08-20
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/126/1/100.pdf
Dhand, Chetna and Sumana, G. and Datta, Monika and Malhotra, B. D. (2010) Electrophoretically deposited nano-structured polyaniline film for glucose sensing. Thin Solid Films, 519 (3). pp. 1145-1150. ISSN 0040-6090 |
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| Relation |
http://dx.doi.org/10.1016/j.tsf.2010.08.059
http://npl.csircentral.net/126/ |
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