Fabrication of Neisseria gonorrhoeae biosensor based on chitosan–MWCNT platform
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Fabrication of Neisseria gonorrhoeae biosensor based on chitosan–MWCNT platform
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| Creator |
Singh, Renu
Sumana, G. Verma, Rachna Sood, Seema Sood, K. N. Gupta, Rajinder K. Malhotra, B. D. |
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| Subject |
Materials Science
Physics |
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| Description |
We report results of studies relating to preparation and characterization of DNA electrodes based on the chitosan–MWCNT/ITO nanobiocomposite platform for electrochemical detection of gonorrhoea, the most common bacterial sexually transmitted disease. (STD). This biosensing electrode has been characterized using FT-IR, SEM, cyclic voltammetry and differential pulse voltammetry, etc. The sensing characteristics have been investigated in phosphate buffer saline using methylene blue as the hybridization indicator. This DNA biosensor has a response time of 60 s and is found to be stable for about 4 months when stored at 4 °C. The results of DPV studies reveal that this bioelectrode can detect complementary DNA concentration in a wide range of 1 × 10− 6 M to 1 × 10− 17 M with a detection limit of 1 × 10− 16 M.
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| Publisher |
Elsevier
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/158/1/131.pdf
Singh, Renu and Sumana, G. and Verma, Rachna and Sood, Seema and Sood, K. N. and Gupta, Rajinder K. and Malhotra, B. D. (2010) Fabrication of Neisseria gonorrhoeae biosensor based on chitosan–MWCNT platform. Thin Solid Films, 519 (3). pp. 1135-1140. ISSN 0040-6090 |
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| Relation |
http://dx.doi.org/10.1016/j.tsf.2010.08.057
http://npl.csircentral.net/158/ |
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